REDDY: PHOSPHORUS LOADING EFFECTS ON ENZYME ACTIVITY ..... at pH. 8.1. This mixture was incubated at 37 C for 1 h. Enzyme activity was stopped by ... The effects of various concentrations of ethylene on enzyme activity ... The enzyme was dissolved in 0.1 M phosphate buffer pH 7.5, at a protein ... The activity of enzymes is strongly affected by changes in pH and temperature. Each enzyme works best at a certain Ehrlich Nissan Volkswagen pH (left graph) and temperature (right ... gai.